Journal: Oncotarget

Article Title: MiR-497 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting VEGFA and AEG-1

doi:

Figure Lengend Snippet: A. The expressions of miR-497 in eight human liver cancer cell lines (HepG2, Huh7, PLC/PRF/5, SMMC-7721, SK-HEP-1, MHCC97-H, MHCC97-L and Hep3B) and two human normal hepatic cell lines (Chang liver and L02) were measured by SYBR Green qRT-PCR. B. miR-497 (measured by SYBR Green qRT-PCR) was down-regulated in HCC tissues compared with the matched adjacent non-tumorous liver tissues. The central horizontal line represents the mean value. C. AEG-1 (measured by SYBR Green qRT-PCR) was up-regulated in HCC tissues compared with the matched adjacent non-tumorous liver tissues. The central horizontal line represents the mean value. D. AEG-1 was inversely correlated with miR-497 expression in the human HCC tissues. E. and F. The expression of CD34 and VEGFA was measured by immunohistochemical staining in the human HCC tissues and matched adjacent non-tumorous liver tissues. HCC: hepatocellular carcinoma tissues; NT: matched adjacent non-tumorous liver tissues; MVD: microvessel density. * P < 0.05.

Article Snippet: The hepatoma cell lines HepG2, Huh7, PLC/PRF/5, SMMC-7721, SK-HEP-1, Hep3B, the human normal hepatic cell line L02 and human umbilical vein endothelial cell lineHUVEC were obtained from the China Center for Type Culture Collection (CCTCC).

Techniques: SYBR Green Assay, Quantitative RT-PCR, Expressing, Immunohistochemical staining, Staining

Journal: Journal of Hepatocellular Carcinoma

Article Title: Coilin Affects the Prognosis of Hepatocellular Carcinoma Through Cell Cycle and Apoptosis

doi: 10.2147/JHC.S500119

Figure Lengend Snippet: Expression of COIL in HCC cell lines and HCC tissue from hospital. The expression of COIL in liver cancer cell lines huh7, hepG2 and hep3B was higher than that in L02 by immunohistochemistry ( A ) and Western blotting ( B ). ( C ) The relative expression of COIL in HCC was significantly higher than that in normal liver tissue by qRT-PCR (***p value < 0.001). ( D ) The expression of COIL in HCC tissue was higher than that in normal liver tissue. ( E, F ) Representative immunohistochemistry results of low or high COIL expression. ( G ) High COIL expression was associated with poor overall survival.

Article Snippet: The human normal liver cell line L02 was obtained from Beijing Baiao Bowei Biotechnology Co., Ltd.

Techniques: Expressing, Immunohistochemistry, Western Blot, Quantitative RT-PCR

Journal: Cell Death & Disease

Article Title: Polyphyllin I suppresses the formation of vasculogenic mimicry via Twist1/VE-cadherin pathway

doi: 10.1038/s41419-018-0902-5

Figure Lengend Snippet: a Polyphyllin I exhibited the most obvious inhibitory effect on VM formation. b Structure of PPI. c Effects of PPI on the viability of SMMC7721, PLC, HepG2, Hep3B, and Bel7402 cells. PPI reduced the survival rate of HCC cells at relatively high concentrations and had slight influence on cell survival below 1 μM. d Cell cycle analysis of PPI in HCC cell lines. 0.1 μM PPI did not influence the cycle of HCC cells. e , f PPI inhibited the migration and invasion of HCC cells. g PPI decreased the number of pipe-like structures in SMMC7721 cells on Matrigel. h Time-lapse images of SMMC7721. PPI inhibited the pipe-like structure formation in SMMC7721 cells ( n = 3)

Article Snippet: HCC cell lines including SMMC7721, PLC, HepG2, Hep3B, and Bel7402 were purchased from Keygene BioTECH (Nanjing, China) and validated through a short tandem repeat-based method.

Techniques: Cell Cycle Assay, Migration